HUNG V. LE, Ph.D. CV Addendum

 

ADDENDUM

PATENTS

  • Mek1 polypeptides: US patent 8,440,443, May 14, 2013
  • Assay for PCSK9 Inhibitors: US Patent 8206943, June 26, 2012
  • Hdm2 Polypeptides: US Patent 7888474, February 15, 2011; US Patent 7705127, April 27, 2010
  • Treatments for Flaviviridae virus infection: US Patent 7,816,339, October 19, 2010; US Patent 7,524,831, April 28, 2009
  • Catalytic domain of ADAM 33 and methods of use thereof: US Patent 7,335,758, February 26, 2008; US Patent 7,208,311, April 24, 2007.
  • Humanized monoclonal antibodies against human interleukin-4: US Patent 5,863,537, January 26, 1999
  • Cloning and expression of humanized monoclonal antibodies against human interleukin-4: US Patent 5,770,403, June 23, 1998
  • Method for preparing zinc interferon alpha-2 crystals: US Patent 5,741,485, April 21, 1998
  • Crystalline r-h-GM-CSF and Method for the preparation: CA Patent 2,058,441, December 16, 1997
  • Polypeptide Inhibitors of gamma Interferon: US Patent 5,632,988, May 27, 1997
  • Crystalline r-h-GM-CSF and method: US Patent 5,616,555, April 1, 1997; US Patent 5,109,119, April 28, 1992.
  • Crystalline Interleukin-4: US Patent, 5,597,900, January 28, 1997
  • Method for preparing interferon alpha-2 crystals: US Patent 5,460,956, October 24, 1995
  • Process for the purification of gamma Interferon: US Patent 4,751,078, June 14, 1988.

PUBLICATIONS

·         Fully-Activated MEK1 Exhibits Compromised Affinity for Binding Of Allosteric Inhibitors U0126 and PD0325901. Payal R. Sheth†*, Yuqi Liu†, Thomas Hesson†, Jia Zhao§, Lev Vilenchik†, Yan-Hui Liu§, Todd W. Mayhood† and Hung V. Le†. (2011) Biochemistry 50 (37), 7964-7966

·         AC-MS methodology for quantitative analyses of small molecule-protein binding in solution. Lev Z. Vilenchik, Payal R. Sheth, Cheng-Chi Chuang & Hung V. Le (2011) Anal. Biochem. 48, 10-18.

·         Evaluation of in vitro PXR-based assays and in silico modeling approaches for understanding the binding of a structurally diverse set of drugs to PXR. Xiao, L., Nickbarg, E., Wang, W., Thomas, A., Ziebell, M., Prosise, W.W., Lesburg, C.A., Taremi, S.S., Gerlach, V.L., Le, H.V. & Cheng, K.-C. (2011) Biochem. Pharmacol. 81, 669-679.

·         Structural basis of CX-4945 binding to human protein kinase CK-2. Andrew D. Ferguson, Payal R. Sheth, Andrea Basso, Smita Paliwal, Kimberly Gray, Thierry O. Fischmann and Hung V. Le (2011) FEBS Lett. 585, 104-110.

·         Novel benzimidazole inhibitors bind to a unique site in the kinesin spindle protein motor domain. Payal R. Sheth, Gerald W. Shipps Jr., Wolfgang Seghezzi, Catherine K. Smith, Cheng-Chi Chuang, David Sanden, D. Allen Annis, Elliott Nickbarg, Yao MA, Brian Lahue, and Hung V. Le (2010) Biochemistry 49, 8350–8358.

·         Expression, purification, stability optimization and characterization of human Aurora B kinase domain from E. coli. Payal R. Sheth, Lata Ramanathan, Ashwin Ranchod, Andrea D. Basso, Rumin Zhang, Kimberly Gray, and Hung V. Le (2010) Archives of Biochemistry & Biophysics 503, 191-201.

·         Purification and Characterization of Truncated Human AMPK <alpha>2<beta>2<gamma>3 Heterotrimer from Baculovirus-Infected Insect Cells. Ramanathan, L., Sheth, P.R., Ogas, P., Xiao, L., Le, H.V. (2010) Protein Expr. Purif. 70, 13-22.

·         Thermodynamics of Nucleotide and Inhibitor Binding to WT and Ispenesib-Resistant Forms of KSP. Sheth, P., Basso, A., Lesburg, C., Duca, J., Ogas, P., Nate, L., Gray, K., Mannarino, A., Prongay, A. & Le, H.V. (2009) Biochemistry 48, 11045-11055.

·         Crystal structures of Mek-1 binary and ternary complexes with nucleotides and inhibitors.Thierry O. Fischmann,1*, Catherine K. Smith1,2*, Joseph Myers, Paul Reichert, Anthony Mannarino, William T. Windsor, Donna Carr, Hugh Zhu, Jesse Wong, Rong-Sheng Yang,  Todd W. Mayhood, Hung V. Le & Vince S. Madison (2009) Biochemistry 48, 2661-2674.

·         Crystal structures of the pro-inflammatory cytokine interleukin-23 and its complex with a high affinity neutralizing antibody. Beyer, Brian M, Ingram, Richard, Ramanathan, Lata, Reichert, Paul, Le, Hung .V., Madison, Vincent. & Orth, Peter. (2008) J. Mol. Biol. 382, 942-955.

·         Construction and characterization of a fully active PXR/SRC-1 tethered protein with increased stability. Wang, Wenyan, Prosise, Winifred, Chen, Jun, Taremi, S, Madison, Vincent, Le, Hung, Cui, Xiaoming, Thomas, Ann, Cheng, Kuo-Chi, Lesburg, Charles (2008) Protein Engineering, Design & Selection 21, 425-433.

·         Structure-Guided Discovery of Cyclin-Dependent Kinase Inhibitors.  Fischmann, T. O,, Hruza, A,, Duca, J.S., Ramanathan, L., Mayhood, T., Windsor, W.T., Le, H.V., Guzi, T.J., Dwyer, M.P., Paruch, K., Doll, R.J., Lees, E., Parry, D., Seghezzi, W. and Madison, V. (2008) Biopolymers (Pept. Sci.) 89, 372-379.

·         Characterization of refolded b-secretase (BACE): Optimization of refolding, removal of propeptide, kinetic characterization and determination of the uncomplexed structure at 2.2 Angstroms. Beyer, B.M., Le, H.V., Madison, V., & Strickland, C. (2006). Manuscript completed, unpublished.

·         Stabilization of the autoproteolysis of TNF-alpha converting enzyme (TACE) results in a novel crystal form suitable for structure-based drug design studies. Ingram, R.N., Orth, P., Strickland, C.L., Le, H.V., Madison, V. & Beyer, B.M. (2006) Protein Engineering, Design & Selection, 19, 155-161.

·         Protease domain of human ADAM33 produced by Drosophila S2 Cells. Prosise, W.W., Yarosh-Tomaine, T., Lozewski, Z., Ingram, R.N., Liu, J.J., Zou, J., Zhu, F.,Taremi, S.S., Hung V. Le & Wang, W. (2004) Protein Expression & Purification, 38, 292-301.

·         Crystallization of glycosylated human BACE expressed in Trichoplusia ni. Wang, W., Reichert, P., Beyer, B., Liu, J., Lee, J., Zhang, L., Liu, Y-H, Taremi, S.S., Le, H.V. & Strickland, C. (2004) Biochim. Biophys. Acta 1698 (2) 255-259.

·         Crystal structure of the catalytic domain of human ADAM 33. Orth, P. Reichert, P., Strickland, C., Hammond, G., Xiao, L., Mirza, U., Le, H.V., Prosise, W.W. Yarosh-Tomaine, T., Wang, W., Taremi, S.S. Zou, J. & Madison, V. (2004) ) J. Mol. Biol. 335, 129-137.

·         Farnesyl Protein Transferase: Identification of K164a and Y300b as catalytic residues by mutagenesis and kinetic studies. Wu, Z, Demma, M., Strickland, C.L., Radisky, E, Le, H.V. & Windsor, W.T. (1999) Biochemistry 38, 11239-11249.

·         Crystallographic and Thermodynamic Analyses of Nonpeptide Tricyclic Inhibitors of Farnesyl Protein Transferase. Strickland, C.L., Weber, P.C., Windsor, W.T.,  Wu, Z., Le, H.V., Albanese, M.M., Alvarez, C.S., Cesarz, D., Rosario, J.D., Deskus, J., Mallams, A.K., Njoroge,F.G., Piwinski, J.J., Remiszewski, S, Rossman, R.R., Traveras, A.G., Vibulbhan, B., Doll, R.J., Girijavallabhan, V.M. and Ganguly, A. K. (1999) J. Med. Chem. 42, 2125-2135.

·         High Level Expression, Purification, Kinetic Characterization and Crystallization of protein farnesyltransferase b-subunit C-Terminal Mutants. Wu, Z., Demma, M., Syto, R., Strickland, C. L., Le, H.V., Weber, P.C. & Windsor W.T. (1999) Protein Eng. 12, 341-348.

·         Emerging Targets in the Treatment of Hepatitis C Virus Infection. Le, H.V., Yao, NH & Weber P.C. (1998) Expert Opinion on Therapeutic Targets 2 (2), 125-136.

·         Crystal Structure of Farnesyl Protein Transferase Complexed with a CaaX peptide and Farnesyl Diphosphate Analogue. Strickland, C., Windsor, W.T., Syto, R., Wang, L., Bond, R., Wu, Z., Schwartz, J., Le, H.V. Beese, L.S. & Weber, P.C. (1998) Biochemistry 37, 16601-16611.

·         The Structure of HCV Helicase and its Relevance to the Design of New Anti-HCV Drugs. Le, H.V., Yao, N-H , Murgolo, N. &  Weber, P.C. (1998) Hepatologia Clinica 6 ( Suppl. 1 ), 77-83.

·         Serine Protease of Hepatitis C Virus Expressed in Insect Cells as the NS3/4A Complex. Sali, D.L., Ingram, R., Wendel, M., Gupta, D., McNemar,C., Tsarbopoulos, A., Chen, J. W., Hong, Z., Chase, R., Risano, C., Zhang, R., Yao, NH, Kwong ,A.D., Ramanathan, L., Hung V. Le and Weber, P.C. (1998) Biochemistry 37, 3392-3401.

·         Mechanism of Autoproteolysis at the NS2-NS3 Junction of the Hepatitis C Virus Polyprotein. Wu, Z., Yao, N.H, Le, H.V. & Weber, P.C. (1998) Trends in Biochemical Sciences 23, 92-93.

·         Probing the substrate specificity of hepatitis C virus serine protease using synthetic peptides. Zhang, R., Durkin, J., Windsor, W. T., McNemar, C., Weber, P. & Le, H.V. (1997) J. Virology 71, 6208-6213.

·         Structure of Hepatitis C virus helicase domain. Yao,N-H, Hesson, T., Cable, M., Hong, Z., Kwong, A., Le. H.V. & Weber, P.C. (1997) Nature Structural Biology 4, 463-467.

·         Inactivation of the human cytomegalovirus protease by diisopropylfluorophosphate. Hesson, T., Tsarbopoulos, A., Taremi, S.S., Prosise, W., Butkiewicz, N., Dasmahapatra, B., Cable, M., Le, H. & Weber, P.C. (1997) Techniques in Protein Chemistry VIII, Marshak, D.R., ed., Academic Press, San Diego, pp 257-266.

·         Thermodynamic and structural analysis of phosphotyrosine polypeptide binding to Grb2-SH2. McNemar, C., Snow, M., Windsor, W.T., Prongay, A., Mui, P., Zhang, R., Durkin, J., Le, H.V., & Weber, P.C. (1997) Biochemistry  36, 10,006-10,014.

·         Small molecule drug screening based on surface plasmon resonance technology. Taremi, S.S., Prosise, W.W., Rajan, Durkin, J., Zhang, R., Windsor & Le, H.V. (1996 ) BIAjournal 3 (1), 21.

·         Human Interleukin 4-Receptor Complex: Neutralization Effect of Two Monoclonal Antibodies. Taremi, S.S., Prosise, W.W., Rajan, N., O'Donnell, R.A. & Le, H.V. (1996) Biochemistry 35, 2322-2331.

·         Characterization of Recombinant Human Interleukin 4 Receptor from CHO Cells: Role of N-linked Oligosaccharides. Rajan, N., Tsarbopoulos, A., Kumarasamy, R., O'Donnell, R., Taremi, S.S., Baldwin, S.W., Seelig, G.F., Fan, X., Pramanik, B. & Le, H.V. (1995) Biochem. Biophys. Res. Commun.  206, 694-702.

·         Immunochemical Mapping of Domains in Human Interleukin 4 Recognized by Neutralizing Monoclonal Antibodies. Ramanathan, L., Ingram, R., Sullivan, L., Greenberg, R., Reim, R., Trotta & Le, H.V. (1993) Biochemistry 32, 3549-3556.

·         Selective Proteolytic Cleavage of Recombinant Human Interleukin 4/ Evidence for a Critical Role of the C-Terminus. Le, H.V., Seelig, G.F., Syto, R., Ramanathan, L., Windsor, W.T., Borkowski, D. & Trotta, P.P. (1991) Biochemistry 30, 9576-9582.

·         Crystallization and Preliminary X-ray Investigation of Recombinant Human Interleukin 4. Cook, W.J., Ealick, S.E., Reichert, P., Le, H.V., Nagabhushan, T.L., Trotta, P.P. & Bugg, C.E. (1991) J. Molec. Biol. 218, 675-678.

·         Purification of Secreted Recombinant Proteins from Escherichia coli. Le, H.V. & Trotta, P.P. (1991) Bioprocess Technol. 12, 163-181.

·         Purification of Secreted Recombinant Proteins from Escherichia coli. Le, H.V. & Trotta, P.P. in:  Purification and Analysis of Recombinant Proteins, Sharma, S.K. & Seetharam, R., eds., Marcel Dekker, Inc., New York, 1991, pp. 163-181.

·         Analysis of the Conformation and Stability of E. coli-Derived Recombinant Human Interleukin 4 by Circular Dichroism. Windsor, W.T., Syto, R. Le, H.V. & Trotta, P.P. (1991) Biochemistry30, 1259-1264.

·         Characterization of Monoclonal Antibodies (MAbs) Directed Against Recombinant Human Interleukin 4. Ramanathan, L., Ingram, R., Abrams, J., Payvandi, F., Trotta, P.P. & Le, H.V. in:  Advances in Gene Technology:  The Molecular Biology of Immune Diseases and the Immune Response, IRL Press, New York 1990, p. 163.

·         Crystallization and Preliminary X-ray Investigation of Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor. Reichert, P., Cook, J.W., Ealick, S.E., Hammond, G.S., Le, H.V., Nagabhushan, T.L., Trotta, P.P. & Bugg, C. (1990) J. Biol. Chem. 265, 452-453.

·         Multiple Forms of Recombinant Murine Interleukin 4 Expressed in COS-7 Monkey Kidney Cells. Ramanathan, L., Le, H.V., Mays-Ichinco, C.A., Syto, R., Arai, N., Nagabhushan, T.L. & Trotta, P.P. (1989) Biochim.  Biophys.  Acta1007, 283-288.

·         Purification and Properties of a Novel Recombinant Human Hybrid Interferon delta-4 alpha-2/alpha-1. Le, H.V., Syto, R., Schwartz, J., Nagabhushan, T.L. & Trotta, P.P. (1988) Biochim.  Biophys.  Acta 957, 143-151.

·         Isolation and Characterization of Multiple Variants of Recombinant Human Interleukin 4 Expressed in Mammalian Cells. Le, H.V., Ramanathan, L., Labdon, J.E., Mays-Ichinco, C.A., Syto, R., Arai, N., Hoy, P., Takebe, Y., Nagabhushan, T.L. & Trotta, P.P. (1988) J. Biol. Chem. 263, 10817-10823.

·         Isolation and Purification of Human Alpha Interferon, a Recombinant DNA Protein. Trotta, P.P., Le, H.V., Sharma, B. & Nagabhushan, T.L. in:  Developments in Industrial Microbiology, Vol. 27, Pierce, G., ed., Elsevier, Amsterdam, 1987, pp. 53-64.

·         Isolation of E. coli-Derived Murine Interleukin-2 from Intracellular and Secretory Expression System. Le, H.V., Syto, R., Mays, C., Reichert, P., Narula, S., Gwain, K., Greenberg, R., Kastelein, R., Van Kimmenade, A., Nagabhushan, T.L. & Trotta, P.P. in:  Protein Purification, Micro to Macro, Burgess, R., ed., Alan R. Liss Inc., NY, 1987, pp. 383-391.

·         Structure-Function Relations in Recombinant Gamma Interferons. Trotta, P.P., Seelig, G.F., Le, H.V., and Nagabhushan, T.L. in: Interferons as Cell Growth Inhibitors and Anti-tumor Factors, Friedman, R., Merigan, T. & Sreevalsan, T., eds, Alan R. Liss, Inc. NY, 1986, pp. 497-507.

·         Structure-Function Relations in Purified Recombinant Murine Gamma Interferon. Le, H.V., Mays, C.A., Syto, R., Nagabhushan, T.L. & Trotta, P.P. in:  The Biology of the Interferon System 1985, Stewart II, W.E., Shellekens, H., eds., Elsevier Science Publishers, 1986, pp. 73-80.

·         Mechanism of Fever Induced by Recombinant Human Interferon. Dinarello, C.A., Bernheim, H.A., Duff, G.W., Le, H.V. Nagabhushan, T.L., Hamilton, N.C. and Coceani, F. (1984) J. Clin. Invest. 74, 906-913.

 POSTERS & INVITED TALKS

·         Key organizer of Continuing Medical Education in Pediatrics (Neonatology & Autism/ADHD) for Hanoi Medical University (June 19-20, 2014), Hue University of Medicine & Pharmacy (June 16, 2015) and University Of Medicine & Pharmacy-HCMC (June 12-13, 2014).

·         Applications of Veterinary Medicine in Drug Discovery. Hung V. Le, Invited Lecture at the Symposium on Veterinary Medicine in a Developing Economy, Faculty of Animal Science & Veterinary Medicine, The University of Agriculture & Forestry, HCMC.

·         Drug Discovery in HCV and HBV. Hung V. Le, Invited Lecture, Faculty of Pharmacy, School of Medicine & Pharmacy, HCMC, VN (May 31, 2012)

·         Aspects of structure-based drug design with HCV NS3 protease. Patenting versus Publications. Hung V. Le, Invited Lectures, Faculty of Biology, University of Science, VNU-Hanoi, VN (May 29, 2012)

·         Approaches to the Development of Medicinal Chemistry: Examples from the drug discovery process for HCV. Strategy for Patenting and Publishing in Drug Discovery. Hung V. Le, Invited Round Table Discussions at the Department of Chemistry, University of Science-VNU-HCMC, VN (May 25, 2012)

·         Drug Discovery in HCV and HBV. Hung V. Le, Invited Lecture, Faculty of Animal Science & Veterinary Medicine, The University of Agriculture & Forestry, HCMC, VN (May 15, 2012)

·         Selected Tools in Protein Science for Drug Discovery. Hung V. Le, Invited Lecture Pfizer-Center for Therapeutic Innovation-New York City (08/26/2011)

·         Applications of thermal denaturation and mass spectrometry in drug discovery. Hung V. Le, invited lecture at KLEPT, The University of Science-Hanoi (05/10/2011), and at The University of Agriculture & Forestry, HCMC, VN (05/04/2011).

·         Discovery Operations in the Pharmaceutical Industry, 1980-2010. Hung V. Le, Invited Lecture at Harvard Medical School, Entrepreneur Club, Boston, MA, January 27, 2011.

·         Engineering constitutively active PXR Ligand Binding Domain for Inverse Structure-Based Design. W. Wang, W. W. Prosise, P. Reichert, X-M Cui*, A. Thomas*, K.-C. Cheng*, L. Xiao, V. Madison, S. S. Taremi, H. V.  Le, C. A. Lesburg. Abstract submitted to the Keystone Symposium on Multi-Protein Complexes in Cell Regulation, St John College, University of Cambridge, Cambridge, UK,, August 18-23, 2006.

·         Production of recombinant insulins & interferons. H. V. Le, Invited lectures, National Institute of Vaccines & Biological Substances, Dalat, VN, (February 14, 2005); Saigon Hi-Tech Park, HCMC, VN (February 16, 2005); Federation of Scientific Societies and Center for Education & Development of Chromatography, HCMC, VN (02-17-2005); Domesco, My Tra- Cao Lanh, VN (February 18, 2005).

·         Structural Biology of the Hepatitis C Virus: Implications for Drug Design. H. V. Le, Invited Lecture, Federation of Scientific Societies and Center for Education & Development of Chromatography, HCMC, VN (02-17-2005).

·         ADAM 33: A novel protease associated with asthma. W. Wang, P. Orth, W.W. Prosise, J. Zou, T. Yarosh-Tomaine, P. Reichert, V. Madison, R. Zhang, L. Xiao, Y-H. Liu, S.S. Taremi & H.V. Le. 19th Enzyme Mechanism Conference, January 5-9, 2005, Asilomar Conference Center, Pacific Grove, CA.

·         The binding of HCV NS3/4A to DNA substrate in the absence of ATP affects the DNA duplex in a manner that facilitates helicase activity, but strand separation requires ATP. Hesson, T. & Le, H.V. FASEB Summer Conference on Helicases: Structure, function and roles in human diseases, June 28-July 3, 2003, Vermont Academy, Saxtons River, VT.

·         Characterization of ADAM 33, a novel metalloprotease implicated in the etiology of asthma. Prosise, W.W., Zou, J., Orth, P., Yarosh-Tomaine, T., Reichert, P., Madison, V., Zhang, R., Xiao, L., Liu, Y-H, Umland, S., Egan, R., Taremi, S.S., Le, H.V. & Wang, W. Gordon Conference on Matrix Metalloproteases, August 17-22, 2003, Big Sky Resort, Big Sky, MT.

·         Characterization of beta-secretase (BACE) from insect cell overexpression: Expression, purification, processing, specificity and crystallization. Brian M. Beyer, Wenyan Wang, Paul Reichert, Corey Strickland, Jim Durkin, Rumin Zhang, Julie Lee, Lilly Zhang, Hung V. Le and Patricia C. Weber. Presented at the Keystone Symposia: Proteolytic Enzymes as Therapeutic Targets, February 3-8, 2002, Keystone, CO.

·         T322 and T324 of HCV helicase are required for energy coupling. H.V. Le. Presented at the 8th International Symposium on Hepatitis C virus & related viruses, September 2-5, 2001, Paris, France.

·         Structure and function of HCV helicase. H.V. Le. Presented at the FASEB Summer Research Conferences: Helicases: Structure, Function & Roles in Human Disease, July 7-12, 2001, Vermont Academy, Saxtons River, VT.

·         T322 and T324 of HCV RNA helicase are required for energy coupling. Hung V. Le, Keith Q. Tanis, Thomas Hesson, Robert Chase, Anita Howe, Zhi Hong, Nanhua Yao and Patricia C. Weber. 17th Enzyme Mechanisms Conference, January 3-6, 2001, Marco Island, FL.

·         Duplex RNA unwinding by the Hepatitis C helicase is fast, processive, and regulated by complex formation with NS4A. Pang, P, Jankowski, E., Le, H.V. & Pyle, A. Presented at RNA 2000, 5th Annual Meeting of the RNA Society, May 30-June 4, 2000, Madison Wisconsin.

·         Tricyclic farnesyl protein transferase inhibitors: Crystallographic and calorimetric studies of structure-activity relationships. C.L. Strickland, P.C. Weber, W.T. Windsor, Z. Wu, H.V. Le, M.M. Albanese, C.S. Alvarez, D. Cezarz, J.D. Rosario, J. Deskus, A.K. Mallams, F.G. Njoroge, J.J. Piwinski, S. Remiszewski, R.R. Rossman, A.G. Taveras, B. Vibulbhan, R.J. Doll, V.M. Giriijavallabhan, and A.K. Ganguly (1999) Presented at the 218th American Chemical Society National Meetibg, New Orleans, LA,  August 22-26.

·         Diversity of the hepatitis C virus genome: Implications for drug therapy and design. H. V. Le (1999) Presented at the 40th Annual Buffalo Medicinal Chemistry Symposium: Drug Resistance: Molecular Mechanism and Intervention Strategies.

·         Screening for inhibitors of HCV NS3 helicase. H.V. Le (1998) Presented at the International Symposium on Hepatitis (November 1-3, 1998). Palm Beach, Florida, USA

·         HCV NS3 protease domain increases the apparent affinity of the NS3 helicase for RNA. Hesson T., Mannarino, A., Cable, M., Taremi, S., Ramanathan, L., Hong, Z., Le, H.V. & Weber, P.C. (1998) The Protein Society Meeting, July 1998, San Diego.

  • The structure of HCV helicase  and its relevance to the design of new anti-HCV drugs. Le, H.V. , Yao, N., Murgolo, N. &  Weber, P.C. (1998) Presented at the 8th International Symposium on viral hepatitis ( January 22-24 ), Madrid, Spain
  • Serine protease in hepatitis C virus NS3-NS4A: Characterization and substrate specificity studies.  Zhang, R., Durkin, J., McNemar, C., Windsor, W.T., Sali-Lipovsek, D., Ramanathan, L., Hesson, T., Cable, M., Kwong, A., Hong, Z. , Yao , N-H, Fischmann, T., Strickland, C., Prongay, A. Weber, P.C.and Le, H.V. (1997) Gordon Research Conference on Enzymes, Coenzymes and Metabolic Parthways, Meriden, NH.
  • Purification and characterization of hepatitis C virus NS3 helicase activity. Chase, R., Yao, N-H, Hesson, T., Lee,  C-G, Ferrari,  E., Wright-Minogue, J., Chen, J., Risano, C., Sali, D., Hong, Z., Cable, M., Le, H.V., Weber, P. C., and Kwong, A.D. (1998) Presented at the 4th International Meeting on Hepatitis C Virus and Related Viruses (March 6-10) , Kyoto, Japan.
  • Inactivation of the human cytomegalovirus protease by diisopropylfluorophosphate. Hesson, T., Tsarbopoulos, A., Taremi, S.S., Prosise, W., Butkiewicz, N., Dasmahapatra, B., Cable, M., Le, H. & Weber, P.C. (1996) The Tenth Symposium of the Protein Society ( San Jose, CA, August 3-7 )
  • Mass spectrometric analysis of the SF9 cell-derived interleukin 5 receptor. Tsarbopoulos, A., Prongay, A., Baldwin, S., Kumarasamy, R., Schwartz, J., Pramanik, Le, H.V., Karas, M. & Hillenkamp, F. (1996)Proceedings of the 44th ASMS Conference (Portland, OR, May 12-16).
  • Characterization of refolded hepatitis C virus-encoded serine protease. Ramanathan, L., Zhang, R., Durkin, J., Wendel, M., Ingram, R., Butkiewicz, N., Jubin, R., Smith, E. B., Murray, M., Das Mahapatra, B., Bishop, R.W., Schwartz, J. & Le, H.V. (1996) Keystone Symposia: Proteolytic Enzymes and Inhibitors in Biology and Medicine (Keystone, CO., March 25-31)
  • Thermodynamics of phosphotyrosine peptide ligand binding to GRB2-SH2 determined by isothermal titration calorimetry. McNemar, C., Prongay, A., Zhang, R., Durkin,  M., Snow, M., Mui, P., Black, S., Wilson, O., Le, H.V., Weber, P.C. & Windsor, W. T. (1996) Biophysical J. 70, A120
  • Recombinant Human Farnesyl Protein Transferase Heterodimer Dissociation Studies. Windsor, W., T., Syto, R., Bond, R., Wang, L., Bishop, R., Schwartz, J., & Le, H.V. (1995)FASEB J.  9, A1436
  • Characterization of Anti IL-5 Receptor Monoclonal Antibodies Using Surface Plasmon Resonance. DeFex, H., Sullivan, L., Le, H. & Prongay, A. (1995) FASEB J.  9, A1422
  • Delineation of Functional Human Interleukin-4 Domains: Mechanisms of Action of Anti-Human IL-4 Neutralizing Antibodies. Taremi, S.S., Prosise, W.W., Durkin, J., Zhang, R. & Le, H.V. BIASymposium 1995, San Diego, CA & Annual Meeting of the International Society for Interferon and Cytokine Research, 1995, Baltimore, MD
  • J. Interferon and Cytokine Research 15, Supp. 1, p. 160
  • Characterization of the receptor binding inhibitory effects of human IL-5 antagonists using surface plasmon resonance. Taremi, S.S., Prosise, W.W., Prosser, D., Wiekowski, M. & Le, H. V. (1995) J. Interferon and Cytokine Research 15, Supp. 1, p. 92
  • Small Molecule Drug Screening Using Surface Plasmon Resonance Technology: Identification of Inhibitors of Cytokine/Cytokine Receptor Interaction. Taremi, S.S., Prosise, W.W., Durkin, J., Zhang, R., Windsor, W.T. & Le, H.V.
  • BIASymposium 1995, Tokyo, Japan
  • Hepatitis C Virus-Encoded Serine Protease: Purification and Enzymatic Characterization of a Novel Construct Expressed in E. Coli. Taremi, S.S., DeFex, H., Prosise, W.W., , Zhang, R., Durkin, J., Mui, P., Butkiewitcz, N., Das Mahapatra, B., Le, H.V., Weber, P.C. & Cable, M. First International Conference on: Therapies for Viral Hepatitis, December 11-15, 1995, Kalapaki Beach, Hawaii.
  • Evaluation of mutant proteins on phage using surface plasmon resonance technology. Taremi, S.S., Prosise, W.W., Fossetta, J., Lunn, C.A., Lundell, D., Cable, M. & Le, H.V. BIASymposium 1994, Baltimore, MD.
  • Protein mapping by matrix-assisted laser desorption/ionization mass spectrometry. Tsarbopoulos, A., Karas, M., Strupat, K., Rajan, N., Le, H.V., Pramanik, B. and Hillenkamp, F. (1994) Proceedings of the 42nd ASMS Conference (Chicago, IL, May 29-June 3)
  • Stoichiometry of IL-4 and Soluble IL-4 Receptor Interaction. Taremi, S.S., Prosise, W.W., Rajan, N., Seelig, G.F., Trotta, P.P., Nagabhushan, T.L., & Le, H.V. (1993) Protein Science 2 (Suppl. 1), 114.
  • Deglycosylation of the Extracellular Domain of Recombinant Human Interleukin 4 Receptor.  Rajan, N., O'Donnell, R., Taremi, S.S., Baldwin, S., Seelig, G.F., Nagabhushan, T.L., Trotta, P.P. & Le, H.V. (1993) Protein Science 2 (Suppl. 1), 114.
  • 1H Sequential Assignments and Secondary Structural Analysis of the C-Terminal Peptide of Interleukin 4. Senior, M.M., Dalgarno, D.C., Slater, B., Mittelman, S., Evans, C.A., Van Le,H., Trotta, P. & Nagabhushan, T.L. (1992) Sixth Symposium of the Protein Society, San Diego, California, July 25-29, Abstract M8.

·         Crystal Structure of Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor. Cook, W.J., Ealick, S.E., Walter,M.R. & Bugg, C.E., Center for Macromolecular Crystallography, University of Alabama

at Birmingham, AL, 35294, and Reichert, P., Hammond, G.S., Le, H.V., Nagabhushan, T.L. & Trotta, P.P., Schering Corporation, Bloomfield, NJ, 07003, ( 1991 ) J. Cell. Biochem. Suppl. 15F, 91

·         Expression and Characterization of a Biologically Active Human Platelet-Derived Growth Factor B. Chain in Escherichia Coli. Dalie, B., Alexander, D., Shanahan, M., Hesson, T., Cable, M., Le, H.V., Trotta, P.P., Zavodny, P., Greenberg, R. & Narula, S.K. (1991) J. Cell.  Biochem. Suppl. 15B, 171 (Abstract D508).

·         Characterization and Structure-Function Relationships in Recombinant Human Interleukin 4 Through Specific Chemical Modification. Windsor, W.T., Syto, R., Durkin, J., Le, H.V., Tindall, S. & Trotta, P.P. (1990) Biophys. J. 57, 423a (Abstract)

·         Disulfide Bond Assignment of Mammalian Cell-Derived Recombinant Human Interleukin 4. Windsor, W.T., Syto, R., Durkin, J., Das, P., Reichert, P., Pramanik, B., Tindall, S., Le, H.V., Labdon, J., Nagabhushan, T.L. & Trotta, P.P. (1990) Biophys. J. 57, 423a (Abstract)

·         Structure-Function Relations in Recombinant Human Interleukin 4:  Effect of Limited Proteolysis. Le, H.V., Zahran, J., Borkowski, B., Syto, R., Ramanathan, L. & Trotta, P.P. (1989) Third Symposium of the Protein Society, Seattle, Washington, July 29-August 2, Abstract T137

·         Conformation and Stability of Recombinant Human Interleukin 4 (huIL-4). Windsor, W., Syto, R., Nagabhushan, T.L., Trotta, P.P. and Le, H.V. (1988) FASEB J. 2, A1337 (Abstract)

·         Characterization of the high Affinity Receptor for Human Interleukin 4 (huIL-4) on Jijoye Cells. Ramanathan, L., Ingram, R., Nagabhushan, T.L., Trotta, P.P. and Le, H.V. (1988) FASEB J. 2, A1650 (Abstract)

·         Isolation of E. coli-Derived Murine Interleukin-2 from Intracellular and Secretory Expression Systems. Le, H.V., Syto, R., Mays, C.A., Reichert, P., Nagabhushan, T.L. & Trotta, P.P. (1987 ) J. Cell  Bioch. , Suppl. 11C, 181 (Abstract).

·         Characterization of Recombinant Human Interleukin 4 derived from COS-7 Monkey Kidney Cells. Le, H.V., Labdon, J.A., Ramanathan, L., Mays, C., Syto, R., Nagabhushan, T.L. & Trotta, P.P. (1987) Fed. Proc. 46, 2230 (Abstract).

·         Novel Pattern of Disulfide Bond Pairing in a Recombinant Hybrid Human Alpha Interferon. Le, H.V., Levine, A.M., Nagabhushan, T.L. & Trotta, P.P. (1986) J. Interfe. Res. 6 Suppl. 1, 75 (Abstract).

·Evidence for the Non-Essentiality of Cysl-Cys98/99 Disulfide Bond in Human Alpha Interferons. Le, H.V., Syto, R., Nagabhushan, T.L. & Trotta, P.P. (1985) Fed. Proc 44, 1398 (Abstract).

·         Structure-Function Relations in Purified Recombinant Murine Gamma Interferon. Le, H.V., Mays, C.A., Syto, R., Nagabhushan, T.L. & Trotta, P.P. in:  The 1985 TNO-ISIR Meeting on the Interferon System, Clearwater Beach, Florida, USA p. 69 (Abstract).

·         Evidence of Similarities between Alpha-4 and Alpha-2 Interferon including Receptor Site Overlap. Seelig, G.F., Schwartz, J., Le, H.V., Trotta, P. & Nagabhushan, T.L. Antiviral Res.-Abstr 1, No. 3 (Special Abstract Issue), 1984, p. 60

·         Effect of Tunicamycin on the Establishment of the Antiviral State by Interferon. Le, H.V., Mahoney, W.C. and Sulkowski, E. (1980) Fed. Proc.  39, 1012 (Abstract).

·         Purification of Human Fibroblast Interferon on Immobilized Polyribonucleotides. Van Le, H., Vastola, K., von Muenchhausen, W. & Sulkowski, E. (1979) J. Clin. Hematolo. Oncol.  9 (4), 273 (Abstract).

·         Action of Partially Thiolated Polynucleotides in Cellular Systems. Le, H.V., Ho, Y.K. & Bardos, T.J. (1978) Proc. Am. Cancer Res. 19, 200 (Abstract).

·         Uptake of Partially Thiolated Nucleic Acids by Tumor Cells. Ho. Y.K., Le, H.V., Paffenholz, V. & Bardos, T.J. (1976) Proc. Am. Ass. Cancer Res. 17, 81 (Abstract).

COMMITTEE APPOINTMENTS

·         Schering-Plough Asian Network & Merck Asia Pacific Network (2008-2010)

·         Schering-Plough President’s Award Reviewer, 2000, 1994-1993, 1991-1990

·         Schering-Plough Antibacterial Task Force, 1994; Chemical Hygiene Committee (1990-1991); Library Committee (1990-1993)

MENTORING

·         Cintyu Wong (University of Rochester, 2002)

·         Shalma Zia Lozewski (Rutgers, 2000-2001)

·         Keith Q. Tanis (New Jersey Institute of Technology, 1998-1999)

·         Henry D. Coleman (University at Buffalo, 1977)

HONORS & AWARDS

·         Schering-Plough Research Institute Excellence Awards (2000, 1994-1992)

·         Schering-Plough Corporation Special Achievement Award (1984)

·         BRSG Institutional Grant Recipient, Roswell Park Memorial Institute (1980)

·         NIH Postdoctoral Fellowship (1977-1978)

·         State University of New York Graduate Fellowship (1974-1977)

·         NIH Graduate Fellowship (1973-1974)

·         University at Buffalo School of Pharmacy Summer Internship (1972 and 1973)

 

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